N- and R-cadherins expression in the rat sciatic nerve demonstrated by postembedding immunogold method on semi-thin sections

Citation
S. Dolapchieva et al., N- and R-cadherins expression in the rat sciatic nerve demonstrated by postembedding immunogold method on semi-thin sections, ANN ANATOMY, 183(5), 2001, pp. 405-411
Citations number
42
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
ISSN journal
0940-9602 → ACNP
Volume
183
Issue
5
Year of publication
2001
Pages
405 - 411
Database
ISI
SICI code
0940-9602(200109)183:5<405:NAREIT>2.0.ZU;2-O
Abstract
The calcium-dependent adhesion molecules Nand R-cadherins take part in the morphogenesis of the nervous system. The data about their localization in t he peripheral nerves postnatum are limited and somewhat controversial. In t he present study, a new methodological approach to the immunohistochemical cadherin detection has been applied. The sciatic nerves of 10-day-old rats were immunotested on semi-thin Epon sections by anti-N- and R-cadherins pol yclonal antibody and visualized by IgG conjugated to colloidal gold and sil ver amplification. In the myelinated nerve fibers, an immuno-like activity was associated with the axoplasm and axolemma, the surface of the myelinati ng Schwann cells and their extracellular matrix. The units of nonmyelinated fibres and their associated glial cells were also intensely immunostained. The myelin sheaths and the glial cytoplasm were immunonegative. The signif icance of the localization is discussed in regard to the possible role of N - and R-cadherins in the anatomical and functional compartmentation of peri pheral nerves.