A. Ohkuchi et al., Expansion of the fraction of Th1 cells in women with preeclampsia: Inversecorrelation between the percentage of Th1 cells and the plasma level of PAI-2, AM J REPROD, 46(4), 2001, pp. 252-259
PROBLEM: The fractions of Th1 cells and Tc1 cells may be increased in preec
lamptic women compared with healthy pregnant women.
METHOD OF STUDY: Eleven healthy non-pregnant women, nine healthy pregnant w
omen (34.1 +/- 3.1 weeks of gestation), and 10 women with preeclampsia (32.
0 +/- 5.4 weeks) were studied. The fractions of Th1 cells, Th2 cells, Tc1 c
ells, and Tc2 cells in the peripheral blood mononuclear cells were determin
ed using a three-color flow cytometric technique. The concentrations of pla
sma plasminogen activator inhibitor-2 (PAI-2) were simultaneously determine
RESULTS: The fraction of Th1 cells was significantly larger in women with p
reeclampsia (18.7 +/- 5.2%) than in normal pregnant women (11.0 +/- 5.7%),
and it increased with a decrease in the PAI-2 level (r = - 0.706, P = 0.002
), which was significantly lower in preeclamptic women (83.4 +/- 46.8 ng/mL
) than in normal pregnant women (225.3 +/- 82.0 ng/mL). The fraction of Tc1
cells increased with increases in the fraction of Th1 cells (r = 0.657, P
< 0.001) and the ratio of Th1-to-Th2 cells (r = 0.535, P = 0.002). The rati
o of Tc1-to-Tc2 cells also increased with an increase in the ratio of Th1-t
o-Th2 cells (r = 0.394, P = 0.031).
CONCLUSIONS: The fraction of Th1 cells appears to be expanded in women with
preeclampsia compared with healthy pregnant women.