An integrated interspecific AFLP map of lettuce (Lactuca) based on two L-sativa x L-saligna F-2 populations

Citation
M. Jeuken et al., An integrated interspecific AFLP map of lettuce (Lactuca) based on two L-sativa x L-saligna F-2 populations, THEOR A GEN, 103(4), 2001, pp. 638-647
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
THEORETICAL AND APPLIED GENETICS
ISSN journal
0040-5752 → ACNP
Volume
103
Issue
4
Year of publication
2001
Pages
638 - 647
Database
ISI
SICI code
0040-5752(200109)103:4<638:AIIAMO>2.0.ZU;2-O
Abstract
AFLP markers were obtained with 12 EcoRI/MseI primer combinations on two in dependent F-2 populations of Lactuca sativa x Lactuca saligna. The polymorp hism rates of the AFLP products between the two different L. saligna lines was 39%, between the two different L sativa cultivars 13% and between the L . sativa and L. saligna parents on average 81%. In both F-2 populations seg regation distortion was found, but only Chromosome 5 showed skewness that w as similar for both populations. Two independent genetic maps of the two F- 2 populations were constructed that could be integrated due to the high sim ilarity in marker order and map distances of 124 markers common to both pop ulations. The integrated map consisted of 476 AFLP markers and 12 SSRs on n ine linkage groups spanning 854 cM. The AFLP markers on the integrated map were randomly distributed with an average spacing between markers of 1.8 cM and a maximal distance of 16 cM. Furthermore, the AFLP markers did not sho w severe clustering. This AFLP map provides good opportunities for use in Q TL mapping and marker-assisted selection.