At-HSP17.6A, encoding a small heat-shock protein in Arabidopsis, can enhance osmotolerance upon overexpression

Sun, WN Bernard, C van de Cotte, B Van Montagu, M Verbruggen, N

Wn. Sun et al., At-HSP17.6A, encoding a small heat-shock protein in Arabidopsis, can enhance osmotolerance upon overexpression, PLANT J, 27(5), 2001, pp. 407-415

36

INGLESE

Article

Plant Sciences","Animal & Plant Sciences

PLANT JOURNAL

0960-7412 → ACNP

27

5

2001

407 - 415

ISI

0960-7412(200109)27:5<407:AEASHP>2.0.ZU;2-#

Owing to their sessile lifestyle, it is crucial for plants to acquire stres s tolerance. The function of heat-shock proteins, including small heat-shoc k proteins (smHSPs), in stress tolerance is not fully explored. To gain fur ther knowledge about the smHSPs, the gene that encoded the cytosolic class II smHSP in Arabidopsis thaliana (At-HSP17.6A) was characterized. The At-HS P17.6A expression was induced by heat and osmotic stress, as well as during seed development. Accumulation of At-HSP17.6A proteins could be detected w ith heat and at a late stage of seed development, but not with osmotic stre ss, suggesting stress-induced post-transcriptional regulation of At-HSP17.6 A expression. Overproduction of At-HSP17.6A could increase salt and drought tolerance in Arabidopsis. The chaperone activity of At-HSP17.6A was demons trated in vitro.