The auxin signal for protoplast swelling is perceived by extracellular ABP1

Steffens, B Feckler, C Palme, K Christian, M Bottger, M Luthen, H

B. Steffens et al., The auxin signal for protoplast swelling is perceived by extracellular ABP1, PLANT J, 27(6), 2001, pp. 591-599

29

INGLESE

Article

Plant Sciences","Animal & Plant Sciences

PLANT JOURNAL

0960-7412 → ACNP

27

6

2001

591 - 599

ISI

0960-7412(200109)27:6<591:TASFPS>2.0.ZU;2-A

Protoplasts of corn coleoptiles and Arabidopsis hypocotyls respond to the p lant hormone auxin with a rapid change in volume. We checked the effect of antibodies directed against epitopes of auxin-binding protein 1 from Arabid opsis thaliana (AtERabp1) and Zea mays (ZmERabp1), respectively. Antibodies raised against the C-terminus of AtERabp1 inhibited the response to auxin, while antibodies raised against a part of box a, the putative auxin-bindin g domain, induced a swelling response similar to that caused by auxin treat ment. Synthetic C-terminal oligopeptides of ZmERabp1 also caused a swelling response. These effects occurred regardless of whether the experiments wer e carried out with homologous (anti-AtERabp1 antibodies on Arabidopsis prot oplasts or anti-ZmERabp1 antibodies in maize protoplasts) or heterologous i mmunological tools. The results indicate that the auxin signal for protopla st swelling is perceived by extracellular ABP1.