The auxin signal for protoplast swelling is perceived by extracellular ABP1

Citation
B. Steffens et al., The auxin signal for protoplast swelling is perceived by extracellular ABP1, PLANT J, 27(6), 2001, pp. 591-599
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
27
Issue
6
Year of publication
2001
Pages
591 - 599
Database
ISI
SICI code
0960-7412(200109)27:6<591:TASFPS>2.0.ZU;2-A
Abstract
Protoplasts of corn coleoptiles and Arabidopsis hypocotyls respond to the p lant hormone auxin with a rapid change in volume. We checked the effect of antibodies directed against epitopes of auxin-binding protein 1 from Arabid opsis thaliana (AtERabp1) and Zea mays (ZmERabp1), respectively. Antibodies raised against the C-terminus of AtERabp1 inhibited the response to auxin, while antibodies raised against a part of box a, the putative auxin-bindin g domain, induced a swelling response similar to that caused by auxin treat ment. Synthetic C-terminal oligopeptides of ZmERabp1 also caused a swelling response. These effects occurred regardless of whether the experiments wer e carried out with homologous (anti-AtERabp1 antibodies on Arabidopsis prot oplasts or anti-ZmERabp1 antibodies in maize protoplasts) or heterologous i mmunological tools. The results indicate that the auxin signal for protopla st swelling is perceived by extracellular ABP1.