An insulator element from the sea urchin Hemicentrotus pulcherrimus suppresses variation in transgene expression in cultured tobacco cells

Citation
S. Nagaya et al., An insulator element from the sea urchin Hemicentrotus pulcherrimus suppresses variation in transgene expression in cultured tobacco cells, MOL GENET G, 265(3), 2001, pp. 405-413
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR GENETICS AND GENOMICS
ISSN journal
1617-4615 → ACNP
Volume
265
Issue
3
Year of publication
2001
Pages
405 - 413
Database
ISI
SICI code
1617-4615(200105)265:3<405:AIEFTS>2.0.ZU;2-L
Abstract
Specialized DNA sequences known as insulators protect genes from both the p ositive and negative influences of nearby chromatin. Many insulators have b een identified in various species; however, few function in multiple specie s. We have shown that an insulator from the Ars (arylsulfatase) gene of the sea urchin Hemicentrotus pulcherrimus functions in plant cells. Normally, expression of an introduced chimeric GUS gene is inactivated in approximate ly 30% of transformed tobacco BY2 clones. Transgenes containing the Ars ins ulator, however, were expressed in all transformed tobacco BY2 cells. The i nsulator did not affect the copy number, the chromosomal position of transg ene integration or maximum expression levels. These results suggest that th e insulator functions to suppress the variation normally associated with tr ansgene expression in tobacco BY2 cells.