A scytalone dehydratase gene from Ophiostoma floccosum restores the melanization and pathogenicity phenotypes of a melanin-deficient Colletotrichum lagenarium mutant

Citation
Hl. Wang et al., A scytalone dehydratase gene from Ophiostoma floccosum restores the melanization and pathogenicity phenotypes of a melanin-deficient Colletotrichum lagenarium mutant, MOL GENET G, 266(1), 2001, pp. 126-132
Citations number
42
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR GENETICS AND GENOMICS
ISSN journal
1617-4615 → ACNP
Volume
266
Issue
1
Year of publication
2001
Pages
126 - 132
Database
ISI
SICI code
1617-4615(200109)266:1<126:ASDGFO>2.0.ZU;2-8
Abstract
Scytalone dehydratase is involved in the production of fungal dihydroxynaph thalene (DHN) melanin. We have isolated and characterized OSD1, a gene enco ding scytalone dehydratase from the sap-staining fungus Ophiostoma floccosu m by PCR-based cloning. Sequence analysis suggests that the OSD1 gene encod es a protein of 216 amino acids with a molecular weight of 24.2 kDa that sh ows 51-70% sequence identity to other scytalone dehydratases. The cloned OS D1 contains two introns of 76 by and 63 by in length, and is the longest sc ytalone dehydratase gene sequence so far reported. Transformation of a DHN melanin-deficient. nonpathogenic, mutant of Colletotrichum lagenarium with the OSD1 gene restored melanin production and pathogenicity. The ability of the mutant to produce the OSD1 gene product was confirmed by RT-PCR analys is. These data show that the cloned OSD1 gene product can function in the D HN melanin biosynthetic pathway in C. lagenarium.