Effects of whole cell sonicates of Treponema lecithinolyticum on osteoclast differentiation

Citation
Bk. Choi et al., Effects of whole cell sonicates of Treponema lecithinolyticum on osteoclast differentiation, J PERIODONT, 72(9), 2001, pp. 1172-1177
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Dentistry/Oral Surgery & Medicine","da verificare
Journal title
JOURNAL OF PERIODONTOLOGY
ISSN journal
0022-3492 → ACNP
Volume
72
Issue
9
Year of publication
2001
Pages
1172 - 1177
Database
ISI
SICI code
0022-3492(200109)72:9<1172:EOWCSO>2.0.ZU;2-L
Abstract
Background: Alveolar bone destruction is a characteristic feature of period ontal diseases and multinucleated osteoclast cells derived from hemopoietic cells are responsible for bone resorption. Treponema lecithinolyticum is a novel oral spirochete isolated from the periodontal lesions. Methods: The effect of whole cell sonicates on the osteoclast differentiati on was examined in a co-culture system of hemopoietic mouse bone marrow cel ls and calvaria derived-osteoblastic cells to clarify the role of T. lecith inolyticum in the alveolar bone destruction associated with periodontal dis eases. The differentiated osteoclasts were confirmed by tartrate-resistant acid phosphatase (TRAP) staining. Results: Sonicates of this bacterium stimulated the osteoclast formation in the co-culture system in a dose-dependent manner. The sonicates-induced os teoclast formation was partially inhibited by the heat treatment of sonicat es. Indomethacin, which is a prostaglandin inhibitor, decreased the osteocl ast formation induced by the bacterial sonicates. Conclusions: These findings suggest that T. lecithinolyticum induces osteoc last differentiation by a prostaglandin E-2-dependent mechanism and that he at-labile components may be involved in this process.