Expression profiling of cancer-related genes in human keratinocytes following non-lethal ultraviolet B irradiation

Citation
T. Murakami et al., Expression profiling of cancer-related genes in human keratinocytes following non-lethal ultraviolet B irradiation, J DERMA SCI, 27(2), 2001, pp. 121-129
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Dermatology
Journal title
JOURNAL OF DERMATOLOGICAL SCIENCE
ISSN journal
0923-1811 → ACNP
Volume
27
Issue
2
Year of publication
2001
Pages
121 - 129
Database
ISI
SICI code
0923-1811(200110)27:2<121:EPOCGI>2.0.ZU;2-N
Abstract
Ultraviolet B irradiation initiates and promotes skin cancers, photo-aging, and immune suppression. In order to elucidate the effect of these processe s at the level of gene expression, we used cDNA microarray technology to ex amine the effect of ultraviolet B irradiation on 588 cancer-related genes i n human keratinocytes at 1, 6, and 24 h post-irradiation with a mildly cyto toxic dose of ultraviolet B (170 mJ/cm(2)). The viability of the irradiated keratinocytes was 75% at 24 h post-irradiation. Various cytokeratins and t ranscription factors were up-regulated within 1 h post-irradiation. After 6 h, expression of a variety of genes related to growth regulation (e.g. p21 (WAFI), notch 4, and smoothened), apoptosis (e.g. caspase 10, hTRIP, and CR AF1), DNA repair (ERCC1, XRCC1), cytokines (e.g. IL-6, IL-13, TGF-beta, and endothelin 2), and cell adhesion (e.g. RhoE, and RhoGDI) were altered in h uman keratinocytes. These data suggest the changes in a cascade of gene exp ression in human keratinocytes occurring within 24 h after UVB exposure. Al though the roles of these cellular genes after UVB-irradiation remain to be elucidated, microarray analysis may provide a new view of gene expression in epidermal keratinocytes following UVB exposure. (C) 2001 Elsevier Scienc e Ireland Ltd. All rights reserved.