Multiple phosphorylated isoforms of NRL are expressed in rod photoreceptors

Citation
Pk. Swain et al., Multiple phosphorylated isoforms of NRL are expressed in rod photoreceptors, J BIOL CHEM, 276(39), 2001, pp. 36824-36830
Citations number
54
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
0021-9258 → ACNP
Volume
276
Issue
39
Year of publication
2001
Pages
36824 - 36830
Database
ISI
SICI code
0021-9258(20010928)276:39<36824:MPIONA>2.0.ZU;2-R
Abstract
NRL, a bZIP transcription factor of the Maf subfamily, interacts with the h omeodomain protein CRX and synergistically regulates rhodopsin expression. Here we report that six isoforms of NRL (29-35 kDa) are generated by phosph orylation and expressed specifically in the mammalian retina. The anti-NRL antibody also crossreacts with a cytosolic 45-kDa protein, which is detecte d in neuronal tissues but is not encoded by the NRL gene. In both human ret inal cell cultures and sections of fetal and adult human retina, NRL is pre sent in the nuclei of developing and mature rods but not cones. We propose that NRL regulates rod photoreceptor-specific gene expression and is involv ed in rod differentiation.