Replication of the rat aldolase B locus differs between aldolase B-expressing and non-expressing cells

Citation
S. Miyagi et al., Replication of the rat aldolase B locus differs between aldolase B-expressing and non-expressing cells, FEBS LETTER, 505(2), 2001, pp. 332-336
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
0014-5793 → ACNP
Volume
505
Issue
2
Year of publication
2001
Pages
332 - 336
Database
ISI
SICI code
0014-5793(20010914)505:2<332:ROTRAB>2.0.ZU;2-B
Abstract
We previously reported a rat chromosomal origin of DNA replication (oriA1) that encompassed the aldolase B (AldB) gene promoter. Here, we examined uti lization of oriA1 in AldB-expressing and non-expressing cells. The results suggested the occurrence of mutually exclusive regulation between DNA repli cation and transcription. Nascent strand abundance as assayed by competitiv e polymerase chain reaction using bromodeoxyuridine-labeled nascent DNA ind icated that oriA1 is not utilized in AldB-expressing cells, while it is fir ed in nonexpressing cells. In the latter non-expressing cells, the replicat ion fork seemed to slow at 20-22 kb downstream of oriA1. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemic al Societies.