Cell cycle-dependent recruitment of HDAC-1 correlates with deacetylation of histone H4 on an Rb-E2F target promoter

Citation
R. Ferreira et al., Cell cycle-dependent recruitment of HDAC-1 correlates with deacetylation of histone H4 on an Rb-E2F target promoter, EMBO REP, 2(9), 2001, pp. 794-799
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
EMBO REPORTS
ISSN journal
1469-221X → ACNP
Volume
2
Issue
9
Year of publication
2001
Pages
794 - 799
Database
ISI
SICI code
1469-221X(200109)2:9<794:CCROHC>2.0.ZU;2-U
Abstract
The transcription factor E2F, which is a key element in the control of cell proliferation, is repressed by Rb and other pocket proteins in growth-arre sted differentiating cells, as well as in proliferating cells when they pro gress through early G(1). It is not known whether similar mechanisms are op erative in the two situations. A body of data suggests that E2F repression by pocket proteins involves class I histone deacetylases (HDACs). It has be en hypothesized that these enzymes are recruited to E2F target promoters wh ere they deacetylate histones. Here we have tested this hypothesis directly by using formaldehyde cross-linked chromatin immunoprecipitation (XChIP) a ssays to evaluate HDAC association in living cells. Our data show that a hi stone deacetylase, HDAC-1, is stably bound to an E2F target promoter during early G, in proliferating cells and released at the G(1)-S transition. In addition, our results reveal an inverse correlation between HDAC-1 recruitm ent and histone H4 acetylation on specific lysines.