Degradation of 1,3-dichloropropene by a soil bacterial consortium and Rhodococcus sp AS2C isolated from the consortium

Citation
Lt. Ou et al., Degradation of 1,3-dichloropropene by a soil bacterial consortium and Rhodococcus sp AS2C isolated from the consortium, BIODEGRADAT, 12(1), 2001, pp. 39-47
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIODEGRADATION
ISSN journal
0923-9820 → ACNP
Volume
12
Issue
1
Year of publication
2001
Pages
39 - 47
Database
ISI
SICI code
0923-9820(2001)12:1<39:DO1BAS>2.0.ZU;2-L
Abstract
A bacterial consortium capable of degrading the fumigant 1,3-D ((Z)- and (E )-1,3-dichloropropene) was enriched from an enhanced soil. This mixed cultu re degraded (Z)- and (E)-1,3-D only in the presence of a suitable biodegrad able organic substrate, such as tryptone, tryptophan, or alanine. After 8 m onths of subculturing at 2- to 3-week intervals, a strain of Rhodococcus sp . (AS2C) that was capable of degrading 1,3-D cometabolically in the presenc e of a suitable second substrate was isolated. (Z)-3-chloroallyl alcohol (3 -CAA) and (Z)-3-chloroacrylic acid (3-CAAC), and (E)-3-CAA and (E)-3-CAAC w ere the metabolites of (Z)- and (E)-1,3-D, respectively. (E)-1,3-D was degr aded faster than (Z)-1,3-D by the strain AS2C and the consortium. AS2C also degraded (E)-3-CAA faster than (Z)-3-CAA. Isomerization of (E)-1,3-D to (Z )-1,3-D or the (Z) form to the (E) form did not occur.