S. Ishihara et al., Intragastric distribution of Helicobacter pylori during short-term omeprazole therapy: study using Carnoy's fixation and immunohistochemistry for detection of bacteria, ALIM PHARM, 15(9), 2001, pp. 1485-1491
Background: There is controversy about the effect of acid-suppressive thera
py on Helicobacter pylori density and the severity of histological gastriti
s in the corpus.
Aim: To evaluate the precise distribution of H. pylori, both on the surface
mucus cells and in the surface mucus gel layer, by using Carnoy's fixation
and immunostaining for the detection of bacteria.
Methods: A total of 19 peptic ulcer patients with H. pylori infection were
studied. All patients received a 6-week course of treatment with omeprazole
(20 mg/day). Before and after the therapy, H. pylori density in Carnoy-fix
ed tissue sections was examined immunohistochemically. The effect of omepra
zole therapy on the severity of gastritis was also evaluated.
Results: H. pylori density and the grade of gastritis significantly decreas
ed in the antrum after omeprazole therapy. In the corpus, however, there we
re no significant changes in H. pylori density or the severity of gastritis
after omeprazole therapy.
Conclusion: Carnoy's fixation and immunostaining was found to be useful for
the detection of H. pylori in the surface mucus gel layer as welt as on th
e surface mucus cells in biopsy tissue sections. By using this method, H. p
ylori density decreased in the antrum, but remained unchanged in the corpus
after a 6-week course of omeprazole therapy.