Adamantylamide dipeptide as effective immunoadjuvant in rabbits and mice

Citation
Pd. Becker et al., Adamantylamide dipeptide as effective immunoadjuvant in rabbits and mice, VACCINE, 19(32), 2001, pp. 4603-4609
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health",Immunology
Journal title
VACCINE
ISSN journal
0264-410X → ACNP
Volume
19
Issue
32
Year of publication
2001
Pages
4603 - 4609
Database
ISI
SICI code
0264-410X(20010914)19:32<4603:ADAEII>2.0.ZU;2-T
Abstract
In the search for more potent and less toxic immunomodulators, adamantylami de dipeptide (AdDP) was synthesized by the covalent union of amantadine wit h the L-alanyl-D-isoglutamine residue of muramyldipeptide (MDP). The presen t experiments demonstrate the ability of AdDP, co-administered with a prote in immunogen, to raise or enhance a humoral response in immunized animals. BALB/c mice were immunized either by the intraperitoneal (ip) or oral route with ovalbumin (Ova) alone or combined with either AdDP or CpG oligonucleo tide (ODN-CpG), a proved adjuvant. A clear adjuvant dose-response relations hip was observed on the increment of Ova-specific serum antibody titers whe n AdDP was used as adjuvant, irrespectively of the administration route. Th e IgG isotype analysis showed that AdDP promotes a consistent increment in IgG1 antibodies associated with a dominant Th2 response pattern. When admin istered by the oral route, AdDP was at least as efficient as ODN-CpG as adj uvant. Similar results were obtained in rabbits immunized by the oral route , suggesting that the adjuvanticity of AdDP is not restricted to the murine system. In conclusion, AdDP was shown to be a powerful and non-toxic adjuv ant at both systemic and mucosal levels, which makes it a promising tool fo r vaccine development. (C) 2001 Elsevier Science Ltd. All rights reserved.