Disposable HSA QCM-immunosensor for practical measurement in liquid

Citation
Sp. Sakti et al., Disposable HSA QCM-immunosensor for practical measurement in liquid, SENS ACTU-B, 78(1-3), 2001, pp. 257-262
Citations number
9
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences","Instrumentation & Measurement
Journal title
SENSORS AND ACTUATORS B-CHEMICAL
ISSN journal
0925-4005 → ACNP
Volume
78
Issue
1-3
Year of publication
2001
Pages
257 - 262
Database
ISI
SICI code
0925-4005(20010830)78:1-3<257:DHQFPM>2.0.ZU;2-P
Abstract
The application of quartz crystal microbalance (QCM) as affinity immunosens or of human serum albumin (HSA) has been investigated. The aim of the inves tigation was the development of a sensor for simple use for physicians in p ractice. The HSA immunosensors were constructed as a disposable immunosenso r based on a cost effective QCM sensor. A self mixing cell without pumping system was constructed together with the QCM-immunosensor for easy handling . The HSA antibody was immobilized on top of a quartz resonator coated with a mixture of polystyrene and p-tert-butyl-calix[8]arene. There were no sig nificant differences between the measured maximum frequency change of off-l ine measurement in air and on-line measurement in PBS. In this experiment, the developed immunosensor allows the measurement of HSA in PBS in the conc entration range from I to 250 mug/mL The limit value concentration of 20 mu g/ml between ill and healthy condition can be well measured. The sensor sho ws a selectivity to HSA in urine. (C) 2001 Elsevier Science B.V. All rights reserved.