Distribution of yieldin, a regulatory protein of the cell wall yield threshold, in etiolated cowpea seedlings

Citation
A. Okamoto-nakazato et al., Distribution of yieldin, a regulatory protein of the cell wall yield threshold, in etiolated cowpea seedlings, PLANT CEL P, 42(9), 2001, pp. 952-958
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT AND CELL PHYSIOLOGY
ISSN journal
0032-0781 → ACNP
Volume
42
Issue
9
Year of publication
2001
Pages
952 - 958
Database
ISI
SICI code
0032-0781(200109)42:9<952:DOYARP>2.0.ZU;2-P
Abstract
We examined the distribution and the immunohistochemical localization of yi eldin in etiolated cowpea seedlings with an anti-yieldin antibody. An immun oblotting analysis revealed that the yieldin was located in the aerial orga ns (plumule, epicotyl and hypocotyl) but not in the roots. The intensity of the yieldin signal in the hypocotyls was highest in the apical pre-elongat ion region (the hook region) and decreased toward the elongated mature base indicating that the yieldin disappeared with the ceasing of cell elongatio n. Tissue-print immunoblotting analysis using hypocotyls in different germi nation stages supports this view because the apical yieldin-rich regions, j ust beneath the cotyledonary node (the hook and rapidly elongating regions) , acropetally migrated together with hypocotyl elongation. Immunohistochemi cal microscopy demonstrated that yieldin was localized in the cell walls of the cortex and epidermis of the germ axes. The present results are consist ent with the view that yieldin participates in the regulation of cell wall yielding during elongation growth.