Identification of oral spirochetes at the species level and their association with other bacteria in endodontic infections

Citation
Iy. Jung et al., Identification of oral spirochetes at the species level and their association with other bacteria in endodontic infections, ORAL SURG O, 92(3), 2001, pp. 329-334
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Dentistry/Oral Surgery & Medicine
Journal title
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS
ISSN journal
1079-2104 → ACNP
Volume
92
Issue
3
Year of publication
2001
Pages
329 - 334
Database
ISI
SICI code
1079-2104(200109)92:3<329:IOOSAT>2.0.ZU;2-C
Abstract
Objectives. Recent molecular approaches have revealed that fastidious organ isms such as Bacteroides forsythus and oral treponemes were frequently foun d in root canals with apical periodontitis. The purpose of this study was t o identify the isolates of oral spirochetes at the species level in endodon tic infections and to determine their association with B forsythus and Porp hyromonas gingivalis. Study design. Seventy-nine teeth with apical periodontitis were selected fo r this study. After sampling from the root canals aseptically, polymerase c hain reaction amplification for the 16S rRNA gene was performed with eubact erial universal primers. Subsequently, dot-blot hybridization was performed with 8 species-specific oligonucleotide probes. The microbial associations were analyzed by using the odds ratio. Results. The most frequently found species was P gingivalis (27.4%), follow ed by Treponema maltophilum (26%), B forsythus (16.4%), and Treponema socra nskii (2.7%). Other treponemes, including Treponema denticola, were not det ected in our samples. Significant microbial associations were identified be tween T maltophilum, B forsythus, and P gingivalis by performing analysis w ith the odds ratio. Conclusion. Our results indicate that T maltophilum should be included in e tiologic studies of endodontic diseases.