Development of sensitive high-performance liquid chromatography with fluorescence detection using 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride as a labeling reagent for determination of bisphenol A in plasma samples

Citation
T. Watanabe et al., Development of sensitive high-performance liquid chromatography with fluorescence detection using 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride as a labeling reagent for determination of bisphenol A in plasma samples, J CHROMAT B, 762(1), 2001, pp. 1-7
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
1387-2273 → ACNP
Volume
762
Issue
1
Year of publication
2001
Pages
1 - 7
Database
ISI
SICI code
1387-2273(20011005)762:1<1:DOSHLC>2.0.ZU;2-S
Abstract
A sensitive HPLC method for determination of bisphenol A (BPA) in plasma sa mples using 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DEB-Cl) as a fluorescence labeling reagent was developed. The fluorescence labeling rea ction was completed within 10 min at room temperature. DEB-Cl reacts with t he phenolic hydroxyl group of BPA in the presence of triethylamine (TEA). T he DIB-Cl derivative of BPA (DIB-BPA) was separated within 30 min with an O DS column using acetonitrile-water (90:10, v/v) as the isocratic eluent, Ca libration graphs were linear over the range of 1.0-100 ng/ml (r=0.999). The detection limit of DIB-BPA was 0.05 ng/ml (2.5 pg) at a signal-to-noise ra tio of 3. The relative standard deviations (RSDs) of the method for between -run were 1.0-5.0%. The analytical recoveries of known amounts (1.0 and 100 ng/ml) of BPA-spiked rabbit plasma were around 95%. (C) 2001 Elsevier Scie nce B.V. All rights reserved.