Separation and determination of podophyllum lignans by micellar electrokinetic chromatography

Citation
Sh. Liu et al., Separation and determination of podophyllum lignans by micellar electrokinetic chromatography, J CHROMAT A, 928(1), 2001, pp. 109-115
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
928
Issue
1
Year of publication
2001
Pages
109 - 115
Database
ISI
SICI code
0021-9673(20010831)928:1<109:SADOPL>2.0.ZU;2-N
Abstract
A micellar electrokinetic chromatography method was established for the qua ntitative analysis of seven podophyllum lignans in Podophyllum emodi Wall. var. chinesis sprague. The optimum buffer system was 10 mM NaH2PO4-5 mM bor ate- 100 mM sodium dodecylsulfate-30% isopropanol (pH 7.20). Voltage was 18 kV and detection at 214 nm. The second derivative chromatogram was used to determine a low-content component and those not fully separated from adjac ent ones. The RSD values of migration times and peak areas were <2.2 and <5 .5%, respectively. The effects of several CE parameters on the resolutions were studied systematically. (C) 2001 Elsevier Science B.V. All rights rese rved.