Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov with Tenacibaculum maritimum comb. nov and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp nov and Tenacibaculum amylolyticum sp nov.

Citation
M. Suzuki et al., Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov with Tenacibaculum maritimum comb. nov and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp nov and Tenacibaculum amylolyticum sp nov., INT J SY EV, 51, 2001, pp. 1639-1652
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN journal
1466-5026 → ACNP
Volume
51
Year of publication
2001
Part
5
Pages
1639 - 1652
Database
ISI
SICI code
1466-5026(200109)51:<1639:PAATSO>2.0.ZU;2-5
Abstract
Bacterial strains were isolated from sponge and green algae which were coll ected on the coast of Japan and Palau. The phylogenetic relationships of th ese isolates among marine species of the Cytophaga-Flavobacterium-Bacteroid es complex were analysed by using their gyrB nucleotide sequences and trans lated peptide sequences (GyrB) in addition to 16S rDNA sequences. These iso lates were closely related to the previously characterized marine Flexibact er species, [Flexibacter] maritimus and [Flexibacter] ovolyticus. These Fle xibacter species are distantly related to Flexibacter flexilis, the type sp ecies of the genus Flexibacter, and phylogenetically belong to the family F lavobacteriaceae (according to analysis using both 16S rDNA and GyrB sequen ces). Their phylogenetic, chemotaxonomic and phenotypic characteristics pro mpted the proposal that these two species should be transferred to the new genus Tenacibaculum, as Tenacibaculum maritimum and Tenacibaculum ovolyticu m, respectively. Two additional new species of the genus Tenacibaculum, Ten acibaculum mesophilum gen. nov., sp. nov. (= MBIC 1140(T) = IFO 16307(T)) a nd Tenacibaculum amylolyticum gen. nov., sp. nov. (= MBIC 4355(T) = IFO 163 10(T)) which were isolated from sponges and macroalgae, are also reported. For taxonomic considerations at the species level, the resolution of gyrB s equences was superior to that of 16S rDNA sequences, and the grouping based on the gyrB phylogram was consistent with DNA-DNA hybridization results.