Effect of cigarette smoke exposure on the modulation of 8-oxo-2 '-deoxyguanosine in rat lungs as analyzed by P-32-postlabeling and HPLC-ECD

Citation
Jm. Arif et al., Effect of cigarette smoke exposure on the modulation of 8-oxo-2 '-deoxyguanosine in rat lungs as analyzed by P-32-postlabeling and HPLC-ECD, INT J ONCOL, 19(4), 2001, pp. 763-766
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN journal
1019-6439 → ACNP
Volume
19
Issue
4
Year of publication
2001
Pages
763 - 766
Database
ISI
SICI code
1019-6439(200110)19:4<763:EOCSEO>2.0.ZU;2-B
Abstract
Cigarette smoke contains several oxidants and free radicals. In the present study, we examined the formation of 8-oxo-2 ' -deoxyguanosine (8-oxodG) in the lungs of female Sprague-Dawley rats exposed to side-stream cigarette s moke for 6 h a day, 7 days a week for 1, 2, 4 and 12 weeks in a whole body- exposure system. The samples were analyzed for 8-oxodG by P-32-postlabeling -TLC enrichment and HPLC-ECD techniques to confirm and compare results. Ani mals were sacrificed 15 h after the cessation of smoke exposure and lung DN A was isolated by phenol/Sevag extractions in the presence of the free radi cal traps, 8-hydroxyquinoline (6.8 mM) and N-t-butyl-alpha -phenyl nitrone (500 muM) to minimize artifactual formation of 8-oxodG during sample work u p. Analysis of lung DNA by P-32-postlabeling-TLC showed 8-oxodG levels (mea n +/- SE) of 1.45 +/-0.24, 2.68 +/-0.65, 2.23 +/-0.28 and 2.93 +/-0.54 per 10(6) nucleotides after 1, 2, 4 and 12 weeks of smoke exposure. The respect ive values in sham-treated rats were 2.76 +/-0.19, 3.69 +/-0.20, 1.44 +/-0. 43 and 2.84 +/-0.45 per 106 nucleotides, suggesting no significant effect o f smoke exposure on tissue levels of 8-oxodG. HPLC-ECD procedure yielded sl ightly higher values for 8-oxodG in all groups, however, again significant differences between sham and smoke-exposed groups were not detected. It is concluded that the chronic exposure to side-stream cigarette smoke does not enhance the formation of 8-oxodG in rat lungs.