Low incidence of genetic alterations of the p16(CDKN2a) in clear cell chondrosarcoma

Citation
Yk. Park et al., Low incidence of genetic alterations of the p16(CDKN2a) in clear cell chondrosarcoma, INT J ONCOL, 19(4), 2001, pp. 749-753
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN journal
1019-6439 → ACNP
Volume
19
Issue
4
Year of publication
2001
Pages
749 - 753
Database
ISI
SICI code
1019-6439(200110)19:4<749:LIOGAO>2.0.ZU;2-D
Abstract
Mutational inactivation of the cyclin-dependent kinase inhibitors (CDKIs) ( p16(CDKN2a)) tumor suppressor gene has been found in a variety of human tum or types. To investigate the involvement of CDKI abnormality in clear cell chondrosarcoma, alterations of CDKIs were examined in clear cell chondrosar coma tissues using a quantitative DNA/PCR, PCR-SSCP. Two of 38 specimens (5 .2%) we analyzed showed abnormally low levels of p16(CDKN2a) amplification, suggesting that the allelic deletion of the gene might be low frequent eve nt in progression of this tumor. For detection of subtle sequence alteratio ns such as point mutations, we performed SSCP analysis of the entire coding region of the pl6(CDKN2a) gene. No altered SSCP patterns were found in 38 clear cell chondrosarcoma specimens. This study reflects the very low incid ence of genetic alterations of the p16(CDKN2a) gene in clear cell chondrosa rcoma. Therefore, we conclude that the alteration of the p16(CDKN2a) gene i s not involved significantly in the development of clear cell chondrosarcom a.