Lactobacillus reuteri LA6 and Lactobacillus gasseri LA39 isolated from faeces of the same human infant produce identical cyclic bacteriocin

Citation
Y. Kawai et al., Lactobacillus reuteri LA6 and Lactobacillus gasseri LA39 isolated from faeces of the same human infant produce identical cyclic bacteriocin, FOOD MICROB, 18(4), 2001, pp. 407-415
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Food Science/Nutrition
Journal title
FOOD MICROBIOLOGY
ISSN journal
0740-0020 → ACNP
Volume
18
Issue
4
Year of publication
2001
Pages
407 - 415
Database
ISI
SICI code
0740-0020(200108)18:4<407:LRLALG>2.0.ZU;2-G
Abstract
Reutericin 6, a bacteriocin produced by Lactobacillus reuteri LA6 that was isolated from the faeces of a human infant at 2 months of age, was purified to homogeneity from broth culture-supernatant by reverse-phase chromatogra phy. Molecular weight (5652) by mass spectrometry and primary structure of reutericin 6 were identical to that of gassericin A produced by Lactobacill us gasseri LA39 which was isolated from the faeces of the same human infant at 4 months old Reutericin 6 was shown to be a class II and cyclic bacteri ocin. PCR amplification on the chromosome DNA of L. reuteri LA6 as a templa te with the primers based on the DNA sequences cloned (gassericin A and aci docin B from Lactobacillus acidophilus M46) and sequencing revealed that L. reuteri LA6 has the structural gene for gassericin A with no variations am ong those primers, These results indicate that a bacteriocin of the same st ructure has been produced by different lactobacilli species isolated from t he same infant. (C) 2001 Academic Press.