Cloning and sequencing of the gene, fmtC, which affects oxacillin resistance in methicillin-resistant Staphylococcus aureus

Citation
H. Komatsuzawa et al., Cloning and sequencing of the gene, fmtC, which affects oxacillin resistance in methicillin-resistant Staphylococcus aureus, FEMS MICROB, 203(1), 2001, pp. 49-54
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
0378-1097 → ACNP
Volume
203
Issue
1
Year of publication
2001
Pages
49 - 54
Database
ISI
SICI code
0378-1097(20010911)203:1<49:CASOTG>2.0.ZU;2-N
Abstract
Two Tn551 insertional mutants with reduced methicillin resistance were isol ated from methicillin-resistant Staphylococcus aureus KSA8. These two mutan ts showed increased susceptibility to P-lactam antibiotics and bacitracin, but not to fosfomycin and vancomycin. Tn551 in these mutants was inserted i nto the same gene, termed fmtC The fmtC gene has an open reading frame of 8 40 amino acid residues with an estimated molecular mass of 96.9 kDa. The N- terminal half of the deduced FmtC protein is very hydrophobic, implying tha t this protein is a membrane-associated protein. (C) 2001 Federation of Eur opean Microbiological Societies. Published by Elsevier Science B.V. All rig hts reserved.