Dynamics of antigen-specific helper T cells at the initiation of airway eosinophilic inflammation

Citation
O. Kaminuma et al., Dynamics of antigen-specific helper T cells at the initiation of airway eosinophilic inflammation, EUR J IMMUN, 31(9), 2001, pp. 2669-2679
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
ISSN journal
0014-2980 → ACNP
Volume
31
Issue
9
Year of publication
2001
Pages
2669 - 2679
Database
ISI
SICI code
0014-2980(200109)31:9<2669:DOAHTC>2.0.ZU;2-G
Abstract
Bronchial asthma is characterized by chronic eosinophilic inflammation of t he bronchial mucosa in which Th2 cells play crucial roles. Ovalbumin-reacti ve Th2 clones were labeled with a fluorescent-probe then infused into unpri med mice to elucidate the dynamics of antigen-specific T cells involved in allergic inflammation. Infiltration of not only labeled antigen-specific T cells, but also unlabeled nonspecific CD4(+) T cells into the bronchial muc osa following inhaled antigen challenge was detectable under confocal micro scopy and flow cytometry Accordingly, labeled T cells in the spleen were de creased, whereas those in hilar lymph nodes were increased upon antigen cha llenge. Approximately 45% of antigen-specific T cells that migrated into th e lungs bore CD25, while another early activation marker, CD69, was express ed on 80% of the migrated T cells. Accordingly, antigen challenge to the mi ce induced in situ proliferation of antigen-specific T cells as well as bro nchial epithelial cells in the lungs. Expression of vascular cell adhesion molecule (VCAM)-1, but not intercellular adhesion molecule (ICAM)-1, on the vascular endothelium in the lungs was enhanced following antigen challenge . Nevertheless, treatment with anti-VCAM-1 antibody, and also anti-ICAM-1 a ntibody strongly suppressed the accumulation of T cells, suggesting that bo th VCAM-1 and ICAM-1 are essential for antigen-stimulated T cell mobilizati on into peripheral tissues. Our current study visualized the kinetics and t he mechanism of antigen-specific T cell migration in response to local chal lenge with a protein antigen.