Human monocyte-derived and CD83(+) blood dendritic cells enhance NK cell-mediated cytotoxicity

Citation
Y. Nishioka et al., Human monocyte-derived and CD83(+) blood dendritic cells enhance NK cell-mediated cytotoxicity, EUR J IMMUN, 31(9), 2001, pp. 2633-2641
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
ISSN journal
0014-2980 → ACNP
Volume
31
Issue
9
Year of publication
2001
Pages
2633 - 2641
Database
ISI
SICI code
0014-2980(200109)31:9<2633:HMACBD>2.0.ZU;2-Q
Abstract
Dendritic cells (DC) are known to be the most potent APC and to stimulate a ntigen-specific T cell responses. Recently it was reported that murine DC w ere also capable of modulating the innate immunity by stimulating NK cells through cell-to-cell contact. In the present study, we examined whether hum an DC could affect NK activity. Both monocyte-derived and CD83(+) blood DC were tested. The addition of DC to cultures of CD56(+) cells resulted in th e significant dose-dependent enhancement of the killing activity against va rious NK-sensitive targets. The resultant activity was comparable to that i nduced by optimal concentrations of various cytokines, including IL-2, IL-1 2, IL-15 and IFN-gamma. Interestingly, DC enhanced the cytotoxicity of CD3( -)CD56(+) NK cells, but not that of CD3(+)CD56(+) T cells. Experiments usin g transwells clearly demonstrated that the enhancement of NK activity by DC was mediated by soluble factors produced by DC. The culture supernatants o f DC also stimulated NK activity. The treatment of both DC and their supern atants with anti-human IL-12 or IL-18 antibodies did not block the enhancem ent of NK cell-mediated cytolysis by DC, indicating that other factor(s) pr oduced by DC were responsible for the enhancement of NK activity. These res ults suggest that human myeloid DC can modulate innate immunity by enhancin g NK activity.