Contribution of double-negative thymic precursors to CD8 alpha alpha(+) intraepithelial lymphocytes of the gut in mice bearing TCR transgenes

Citation
D. Guy-grand et al., Contribution of double-negative thymic precursors to CD8 alpha alpha(+) intraepithelial lymphocytes of the gut in mice bearing TCR transgenes, EUR J IMMUN, 31(9), 2001, pp. 2593-2602
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
ISSN journal
0014-2980 → ACNP
Volume
31
Issue
9
Year of publication
2001
Pages
2593 - 2602
Database
ISI
SICI code
0014-2980(200109)31:9<2593:CODTPT>2.0.ZU;2-3
Abstract
Using male and female RAG(-/-) mutant mice expressing TCR transgenes specif ic for MHC class I- or II-presented HY peptides, we performed quantitative and phenotypic comparisons between the TCR lymphocytes present in the lymph oid organs and the gut mucosa in euthymic versus athymic (nude) animals. Th ese comparisons suggest that only a minority of the TCR+ CD8 alpha alpha () intraepithelial lymphocytes (IEL) of the transgenic euthymic mice origina te from hematopoietic precursors acquiring a TCR in the gut wall, while a m ajority of these CD8 alpha alpha (+) IEL appear to be of thymic origin (as were all TCR+ CD8 alpha beta (+) or CD4(+) in any location); these last cel ls are released from the thymus as double-negative thymocytes, which are at a more immature stage (CD44(+)CD25(+)) in female mice than in males (CD44( -)). In view of previous observations that in non-transgenic athymic mice t he CD8 alpha alpha +TCR+ IEL populations are also markedly reduced quantita tively, the possibility of a thymic contribution to these ontogenically pec uliar populations may also exist in normal mice. At which stage of differen tiation such precursors might leave the thymus of normal adult mice remains to be explored.