Transcriptional reprogramming of dendritic cells by differentiation stimuli

Citation
F. Granucci et al., Transcriptional reprogramming of dendritic cells by differentiation stimuli, EUR J IMMUN, 31(9), 2001, pp. 2539-2546
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
ISSN journal
0014-2980 → ACNP
Volume
31
Issue
9
Year of publication
2001
Pages
2539 - 2546
Database
ISI
SICI code
0014-2980(200109)31:9<2539:TRODCB>2.0.ZU;2-J
Abstract
Immature and mature dendritic cells (DC) have been well characterized funct ionally and phenotypically. Microorganisms or bacterial products such as li popolysaccharide (LIPS) and inflammatory molecules, including tumor necrosi s factor (TNF-alpha), are both believed to activate the DC maturation progr am which allows DC to initiate and amplify innate and adaptive immune respo nses. However, there is increasing evidence that the functional state of DC , induced by different stimuli, may be relevant for the immune response out come. Thus, we compared the transcriptional program of mature, transitional and immature DC, after either LPS or TNF-alpha stimulation. GeneChip(R) ol igonucleotide microarrays, representing approximately 6,500 murine genes an d ESTs, were used for this analysis. A very diverse modulation of gene expr ession was observed with the two stimuli. Only LPS-treated cells showed a p attern of expression of genes compatible with a definitive growth arrest an d with a suitable activation and control of the immune response.