A. Ghadersohi et Ak. Sood, Prostate epithelium-derived ets transcription factor mRNA is overexpressedin human breast tumors and is a candidate breast tumor marker and a breasttumor antigen, CLIN CANC R, 7(9), 2001, pp. 2731-2738
Purpose: There is a need to identify novel breast tumor-associated molecule
s with a potential as diagnostic/prognostic markers of breast cancer as wel
l as targets of vaccine and drug discovery against this cancer.
Experimental Design: We used a combination of digital differential display
and reverse transcription-PCR (RT-PCR) methods to identify breast tumor-ass
Results: It was found that prostate epithelium-derived Ets transcription fa
ctor (PDEF) and five other cDNAs occur at high frequency in the cDNA librar
ies from normal human breast tissue and human breast tumors. In contrast, t
hese cDNAs are either undetectable or present at low frequencies in the cDN
A libraries from other normal human tissues. RT-PCR expression analysis of
PDEF showed it to be overexpressed in 14 of 20 primary human breast tumors
and in one metastases tested. Also, consistent with the digital differentia
l display data, RT-PCR analysis of PDEF expression showed highly restricted
expression in normal human tissues. Furthermore, we show that PDEF transcr
ipt levels are 192-fold higher in the peripheral blood of a breast cancer p
atient in comparison with two normal individuals and another breast cancer
patient. In contrast to PDEF, RT-PCR analysis of the expression of the othe
r three cDNAs, including MYL5, Hs.44017, and Hs.215937, showed that these c
DNAs are expressed in several normal human tissues.
Conclusions: These results suggest that PDEF is a breast tumor-associated c
DNA and should be further evaluated for its potential as a breast tumor mar
ker and a breast tumor antigen.