Prostate epithelium-derived ets transcription factor mRNA is overexpressedin human breast tumors and is a candidate breast tumor marker and a breasttumor antigen

Citation
A. Ghadersohi et Ak. Sood, Prostate epithelium-derived ets transcription factor mRNA is overexpressedin human breast tumors and is a candidate breast tumor marker and a breasttumor antigen, CLIN CANC R, 7(9), 2001, pp. 2731-2738
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
Journal title
CLINICAL CANCER RESEARCH
ISSN journal
1078-0432 → ACNP
Volume
7
Issue
9
Year of publication
2001
Pages
2731 - 2738
Database
ISI
SICI code
1078-0432(200109)7:9<2731:PEETFM>2.0.ZU;2-W
Abstract
Purpose: There is a need to identify novel breast tumor-associated molecule s with a potential as diagnostic/prognostic markers of breast cancer as wel l as targets of vaccine and drug discovery against this cancer. Experimental Design: We used a combination of digital differential display and reverse transcription-PCR (RT-PCR) methods to identify breast tumor-ass ociated cDNAs. Results: It was found that prostate epithelium-derived Ets transcription fa ctor (PDEF) and five other cDNAs occur at high frequency in the cDNA librar ies from normal human breast tissue and human breast tumors. In contrast, t hese cDNAs are either undetectable or present at low frequencies in the cDN A libraries from other normal human tissues. RT-PCR expression analysis of PDEF showed it to be overexpressed in 14 of 20 primary human breast tumors and in one metastases tested. Also, consistent with the digital differentia l display data, RT-PCR analysis of PDEF expression showed highly restricted expression in normal human tissues. Furthermore, we show that PDEF transcr ipt levels are 192-fold higher in the peripheral blood of a breast cancer p atient in comparison with two normal individuals and another breast cancer patient. In contrast to PDEF, RT-PCR analysis of the expression of the othe r three cDNAs, including MYL5, Hs.44017, and Hs.215937, showed that these c DNAs are expressed in several normal human tissues. Conclusions: These results suggest that PDEF is a breast tumor-associated c DNA and should be further evaluated for its potential as a breast tumor mar ker and a breast tumor antigen.