Interleukin 8 is an autocrine growth factor and a surrogate marker for Kaposi's sarcoma

Citation
R. Masood et al., Interleukin 8 is an autocrine growth factor and a surrogate marker for Kaposi's sarcoma, CLIN CANC R, 7(9), 2001, pp. 2693-2702
Citations number
59
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
Journal title
CLINICAL CANCER RESEARCH
ISSN journal
1078-0432 → ACNP
Volume
7
Issue
9
Year of publication
2001
Pages
2693 - 2702
Database
ISI
SICI code
1078-0432(200109)7:9<2693:I8IAAG>2.0.ZU;2-3
Abstract
Kaposi's sarcoma (KS) is the most common tumor associated with HIV-1 infect ion. Here, we report the expression, regulation, and biological effect of i nterleukin (IL)-8 in KS. AIDS-KS cell lines expressed higher levels of IL-8 than either human umbilical vein endothelial cells (HUVECs), human aortic smooth muscle (AoSM) cells or fibroblast cells (T1). The inflammatory cytok ine IL-1 beta up-regulated IL-8 expression in a time- and concentration-dep endent manner in KS cell lines. IL-8 antisense oligonucleotides specificall y reduced IL-8 mRNA and protein levels and inhibited KS cell growth in a do se-dependent manner. In addition, supernatant from a KS cell line induced t he growth of HUVECs and angiogenesis in chicken chorioallantoic membrane as says, both of which were inhibited by IL-8 neutralizing antibody. Serum lev els of IL-8 were also elevated in KS cases compared with matched controls. Modulation of IL-8 may thus be of therapeutic value in this disease.