Kaposi's sarcoma (KS) is the most common tumor associated with HIV-1 infect
ion. Here, we report the expression, regulation, and biological effect of i
nterleukin (IL)-8 in KS. AIDS-KS cell lines expressed higher levels of IL-8
than either human umbilical vein endothelial cells (HUVECs), human aortic
smooth muscle (AoSM) cells or fibroblast cells (T1). The inflammatory cytok
ine IL-1 beta up-regulated IL-8 expression in a time- and concentration-dep
endent manner in KS cell lines. IL-8 antisense oligonucleotides specificall
y reduced IL-8 mRNA and protein levels and inhibited KS cell growth in a do
se-dependent manner. In addition, supernatant from a KS cell line induced t
he growth of HUVECs and angiogenesis in chicken chorioallantoic membrane as
says, both of which were inhibited by IL-8 neutralizing antibody. Serum lev
els of IL-8 were also elevated in KS cases compared with matched controls.
Modulation of IL-8 may thus be of therapeutic value in this disease.