Comprehensive allelotyping of human intrahepatic cholangiocarcinoma

Citation
H. Momoi et al., Comprehensive allelotyping of human intrahepatic cholangiocarcinoma, CLIN CANC R, 7(9), 2001, pp. 2648-2655
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
Journal title
CLINICAL CANCER RESEARCH
ISSN journal
1078-0432 → ACNP
Volume
7
Issue
9
Year of publication
2001
Pages
2648 - 2655
Database
ISI
SICI code
1078-0432(200109)7:9<2648:CAOHIC>2.0.ZU;2-R
Abstract
We performed a genome-wide scan for loss of heterozygosity (LOH) in 22 intr ahepatic cholangiocarcinoma (ICC) cases using 168 polymorphic microsatellit e markers throughout all of the human chromosomes and 48 markers of which L OH is reportedly characteristic of hepatocellular carcinoma (HCC). Markers with LOH in more than 30% of informative cases were observed at 21 loci. Am ong these, eight markers on 6q (three loci), 4q (two loci), 9q, 16q, and 17 p shared high frequencies of LOH with HCC in our previous study. As for gro ss appearance, mass-forming type tumors showed higher frequency of LOH (P < 0.001) compared with other types. Compared by tumor size (less than or equ al to5 cm versus >5 cm), number (multiple versus solitary), and the Interna tional Union Against Cancer TNM classification (stage IVB versus II-IVA), L OH was observed more frequently in advanced stages (P < 0.01, respectively) . However, LOH frequency does not differ regardless of lymph node status (p N0 versus pN1). Frequent LOH on 1p36 including the p73 locus was noted in l arge tumors without lymph node metastasis. These suggest that ICC shares so me common carcinogenic steps with HCC such as LOH of 4q and 6q and that ina ctivation of tumor suppressor genes on chromosome 1p36 contributes to progr ession of ICC but not to metastatic traits.