Eukaryotic expression plasmid transfer from the intracellular bacterium Listeria monocytogenes to host cells

Citation
M. Hense et al., Eukaryotic expression plasmid transfer from the intracellular bacterium Listeria monocytogenes to host cells, CELL MICROB, 3(9), 2001, pp. 599-609
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
CELLULAR MICROBIOLOGY
ISSN journal
1462-5814 → ACNP
Volume
3
Issue
9
Year of publication
2001
Pages
599 - 609
Database
ISI
SICI code
1462-5814(200109)3:9<599:EEPTFT>2.0.ZU;2-T
Abstract
The facultative intracellular, Gram-positive bacterium Listeria monocytogen es invades phagocytic and non-phagocytic cells from the tissues and organs of a wide variety of animals and humans. Here, we report the use of these b acteria as vehicles for gene transfer. Eukaryotic expression plasmids, were introduced into the nucleus of host cells following lysis of the intracyto solic, plasm id-carrying bacteria with antibiotics. Cell lines of different tissues and species could be transfected in this way. We examined bacteria l properties required for delivery of the expression plasmids. and found th at this was strictly dependent on the ability of these bacteria to both inv ade eukaryotic cells and egress from the vacuole into the cytosol of the in fected host cells. Macrophagelike cell lines or primary, peritoneal macroph ages proved to be almost refractory to Listeria-mediated gene transfer. Thu s, attenuated L. monocytogenes represents a serious candidate for considera tion as a DNA-transfer vehicle for in vivo somatic gene therapy. The potent ial for oral administration of L. monocytogenes and the ease in producing a nd cultivating recombinant strains are further attributes that make its use as a gene transfer vehicle attractive.