Arginase expression in peritoneal macrophages and increase in circulating polyamine levels in mice infected with Schistosoma mansoni

Citation
Oms. Abdallahi et al., Arginase expression in peritoneal macrophages and increase in circulating polyamine levels in mice infected with Schistosoma mansoni, CELL MOL L, 58(9), 2001, pp. 1350-1357
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
CELLULAR AND MOLECULAR LIFE SCIENCES
ISSN journal
1420-682X → ACNP
Volume
58
Issue
9
Year of publication
2001
Pages
1350 - 1357
Database
ISI
SICI code
1420-682X(200108)58:9<1350:AEIPMA>2.0.ZU;2-0
Abstract
We investigated the nitric oxide (NO) synthase and arginase pathways in res ident peritoneal macrophages of mice infected with the tropical parasite Sc histosoma mansoni. The two enzymes may have opposite effects, insofar as NO may be involved in the killing of the parasite whereas arginase may stimul ate parasite growth via polyamine synthesis. We determined the effects of t he infection on the expression and activity of the two enzymes in macrophag es, before and after cytokine activation. Cells from infected mice expresse d the hepatic type I arginase, whereas in control cells, the enzyme was exp ressed only after cytokine activation, as were NO synthase II and type II a rginase in both groups of cells. Moreover, we found that in infected mice, arginase expression in macrophages was associated with a ten fold increase in the concentration of circulating ornithine-derived polyamines. This may be of pathological importance, since parasitic helminths are though to be d ependent on their hosts for the uptake and interconversion of polyamines.