Peroxisomes in the apocrine sweat glands of the human axilla and their putative role in pheromone production

Citation
G. Rothardt et K. Beier, Peroxisomes in the apocrine sweat glands of the human axilla and their putative role in pheromone production, CELL MOL L, 58(9), 2001, pp. 1344-1349
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
CELLULAR AND MOLECULAR LIFE SCIENCES
ISSN journal
1420-682X → ACNP
Volume
58
Issue
9
Year of publication
2001
Pages
1344 - 1349
Database
ISI
SICI code
1420-682X(200108)58:9<1344:PITASG>2.0.ZU;2-J
Abstract
The products of the human apocrine axillary glands contain volatile steroid s which act as pheromones, The steroidal structure of these pheromones impl ies that the axillary glands should be able to synthesize cholesterol which is the essential precursor of these molecules. Since important steps in ch olesterol synthesis are localized within peroxisomes, we investigated the o ccurrence and the putative role of peroxisomes in the axillary glands at pr otein and mRNA levels by immunocytochemistry, Western blotting, and RT-PCR. Numerous peroxisomes were localized in the cells of the apocrine glands by immunocytochemistry, and the presence of catalase was confirmed by Western blotting and RT-PCR. Additionally, RT-PCR revealed the presence of mRNAs o f two peroxisome-associated enzymes of the cholesterol biosynthetic pathway , mevalonate kinase and farnesyl diphosphate synthase, The results suggest that the peroxisomes in the human apocrine axillary glands may play a pivot al role in the biosynthesis of pheromones.