Recombinant expression of perchloric acid-soluble protein reduces cell proliferation

Citation
H. Kanouchi et al., Recombinant expression of perchloric acid-soluble protein reduces cell proliferation, CELL MOL L, 58(9), 2001, pp. 1340-1343
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
CELLULAR AND MOLECULAR LIFE SCIENCES
ISSN journal
1420-682X → ACNP
Volume
58
Issue
9
Year of publication
2001
Pages
1340 - 1343
Database
ISI
SICI code
1420-682X(200108)58:9<1340:REOPAP>2.0.ZU;2-L
Abstract
Perchloric acid-soluble protein (PSP) may play an important role in the reg ulation of cellular physiological functions because it has been highly cons erved throughout evolution; however, this role has not been well elucidated . In previous reports, we suggested that PSP regulates cell proliferation. In this study, we examined the effect of PSP expression on proliferation of the normal rat kidney cell line NRK-52E, the rat hepatocyte cell line RLN- 10, and the rat hepatoma cell line dR-Lh-84. Cells transfected with pcDNA-s ense-PSP (pcDNA-S-PSP) over-expressed PSP mRNA and protein, and cell prolif eration of the transfected cells was suppressed compared with that of cells transfected with pcDNA-empty (pcDNA-E). Cell viability of pcDNA-S-PSP-tran sfected cells was similar to that of pcDNA-E-transfected cells. Thus, over- expression of PSP suppresses cell proliferation without any influence on ce ll viability. These findings are the first to report an inhibitory activity of PSP on cell proliferation.