Production of butyrylcholinesterase by Caco-2 cells: lack of relationship with triglyceride production

Citation
Ew. Randell et al., Production of butyrylcholinesterase by Caco-2 cells: lack of relationship with triglyceride production, CELL MOL L, 58(9), 2001, pp. 1327-1332
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
CELLULAR AND MOLECULAR LIFE SCIENCES
ISSN journal
1420-682X → ACNP
Volume
58
Issue
9
Year of publication
2001
Pages
1327 - 1332
Database
ISI
SICI code
1420-682X(200108)58:9<1327:POBBCC>2.0.ZU;2-A
Abstract
Elevated levels of butyrylcholinesterase activity occur under a number of h ypertriglyceridemic conditions, including diabetes and obesity. This study examines whether butyrylcholinesterase activity has a direct effect on trig lyceride production, using Caco-2 cells, a human intestinal adenocarcinoma cell line. Caco-2 cells were incubated with 500 muM oleate to stimulate tri glyceride production, and butyrylcholinesterase activity was measured in th e cellular homogenate. Butyrylcholinesterase activity was approximately 3x1 0(-3) mu mol/min per milligram protein. Although triglyceride production in creased by almost five-fold after 18 h of stimulation with oleate, butyrylc holinesterase activity was not increased. Furthermore, inhibition of butyry lcholinesterase activity using 1 mM tetraisopropylpyrophosphoramide did not significantly affect triglyceride production or secretion. Human insulin ( 100 muU/ml) increased the production of butyrylcholinesterase without incre asing triglyceride production. This demonstrates that stimulation of fatty acid production and butyrylcholinesterase activity occur by independent mec hanisms and suggests that their correlation in hyperlipidemic conditions is not due to a direct relationship in production in situ.