Measurement of F-2-isoprostanes unveils profound oxidative stress in aged rats

Citation
Lj. Roberts et Jf. Reckelhoff, Measurement of F-2-isoprostanes unveils profound oxidative stress in aged rats, BIOC BIOP R, 287(1), 2001, pp. 254-256
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
287
Issue
1
Year of publication
2001
Pages
254 - 256
Database
ISI
SICI code
0006-291X(20010914)287:1<254:MOFUPO>2.0.ZU;2-2
Abstract
Free radicals have been theorized to play a causative role in the normal ag ing process. To date, methods used to detect oxidative stress in aged exper imental animals have only detected 2- to 3-fold differences or less between young and aged animals. Measurement of F-2-isoprostanes has emerged as pro bably the most reliable approach to assess oxidative stress status in vivo. Therefore, we measured levels of F-2-isoprostanes free in plasma and level s esterified in plasma lipids in young rats (3-4 months of age) and aged ra ts (22-24 months of age). Plasma concentrations of free F-2-isoprostanes we re increased dramatically by a mean of 20.3-fold (range 4.3 to 42.9-fold) a nd levels esterified in plasma lipids were also strikingly increased by a m ean of 29.9-fold (range 15.8 to 50.0-fold). These findings unveil profound oxidative stress in aged rats which adds considerable support for the free radical theory of aging. (C) 2001 Academic Press.