Y. Hamamoto et al., Recovery of function and mass of endogenous beta-cells in streptozotocin-induced diabetic rats treated with islet transplantation, BIOC BIOP R, 287(1), 2001, pp. 104-109
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Islet transplantation corrects chronic hyperglycemia by augmentation of ins
ulin supply from the graft tissue, but the role of endogenous beta -cells a
fter transplantation is not clear. In the present study, we examined endoge
nous beta -cell function after glucose homeostasis had been reestablished b
y islet graft in streptozotocin (STZ)-induced diabetic rats. Fed plasma glu
cose levels in diabetic rats transplanted with a large number of islets (25
00 islets) into the left kidney capsule soon became lower (139.8 +/- 8.2 mg
/dl) and close to the level in controls (129.7 +/- 11.3 mg/dl), and IPGTT e
xhibited a pattern of plasma glucose response almost identical to control.
The insulin and DNA contents, islet area, and the distribution of beta -cel
ls that were markedly deteriorated in islets of STZ rats were significantly
restored in transplanted rats. The insulin release in response to glucose
or alpha -ketoisocaproate was less in STZ rats, while in islets of transpla
nted rats the secretion recovered to levels similar to controls. On the oth
er hand, arginine-induced insulin release was conversely hyperresponsive in
STZ rats, but in transplanted rats, the response was decreased similar to
controls. Thus, as the plasma glucose level normalizes, residual beta -cell
s show a recovery of function that cannot be accounted for by the increase
in mass alone. (C) 2001 Academic Press.