Flowering plant sperm contains a cytosolic soluble protein factor which can trigger calcium oscillations in mouse eggs

Citation
St. Li et al., Flowering plant sperm contains a cytosolic soluble protein factor which can trigger calcium oscillations in mouse eggs, BIOC BIOP R, 287(1), 2001, pp. 56-59
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
287
Issue
1
Year of publication
2001
Pages
56 - 59
Database
ISI
SICI code
0006-291X(20010914)287:1<56:FPSCAC>2.0.ZU;2-L
Abstract
There is evidence showing that the sperm-induced Ca2+ oscillations in mamma lian eggs at fertilization are triggered by a sperm-derived protein factor. It was established recently that the activity of the putative sperm protei n in causing Ca2+ oscillations in mammalian eggs is not species-specific in vertebrates (1, 16). Here we report that cytosolic soluble extracts derive d from flowering plant sperms in Brassica campestris can also induce fertil ization-like Ca2+ oscillations when microinjected into mouse eggs. The fact or responsible for inducing Ca2+ oscillations in the plant sperm was sperm- specific and heat- or trypsin-labile. Eight to ten sperm equivalents of the plant sperm extracts had enough activity to trigger Ca2+ oscillations in m ouse eggs. Our study suggests that, although plant and mammal are evolution ary divergent species, the activity of the putative sperm protein factor in triggering Ca2+ signaling in mammalian eggs is not specific to the animal kingdom. (C) 2001 Academic Press.