Molecular characterization and heterologous expression of hypCD, the firsttwo [NiFe] hydrogenase accessory genes of Thermococcus litoralis

Citation
M. Takacs et al., Molecular characterization and heterologous expression of hypCD, the firsttwo [NiFe] hydrogenase accessory genes of Thermococcus litoralis, ARCH MICROB, 176(3), 2001, pp. 231-235
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF MICROBIOLOGY
ISSN journal
0302-8933 → ACNP
Volume
176
Issue
3
Year of publication
2001
Pages
231 - 235
Database
ISI
SICI code
0302-8933(200109)176:3<231:MCAHEO>2.0.ZU;2-A
Abstract
The hypCD genes, encoding the counterparts of mesophilic proteins involved in the maturation of [NiFe] hydrogenases, were isolated from the hypertherm ophilic archaeon Thermococcus litoralis. The deduced gene products showed 3 0-40% identity to the corresponding mesophilic proteins. HypC and HypD were synthesized by the T7 expression system. Heterologous complementation expe riments were done in Escherichia coli and Ralstonia eutropha strains lackin g functionally active hypC and hypD genes. Only the cytoplasmic hydrogenase of R. eutropha could be processed by HypD from T litoralis. This was the f irst demonstration of mesophilic hydrogenase processing using a hyperthermo philic archaeal accessory protein to produce an active enzyme.