Effect of diethyldithiocarbamate on proliferation, redifferentiation, and apoptosis of human hepatoma cells

Citation
Jh. Kang et al., Effect of diethyldithiocarbamate on proliferation, redifferentiation, and apoptosis of human hepatoma cells, ACT PHAR SI, 22(9), 2001, pp. 785-792
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
ACTA PHARMACOLOGICA SINICA
ISSN journal
0253-9756 → ACNP
Volume
22
Issue
9
Year of publication
2001
Pages
785 - 792
Database
ISI
SICI code
0253-9756(200109)22:9<785:EODOPR>2.0.ZU;2-Z
Abstract
METHODS: Cell surface charge, biochemical changes, cell growth in soft agar , single cell electrophoresis, electron microscopy examination, and flow cy tometry analysis were measured. RESULTS: After being treated with DDC 3 mmo l/L the growth curve and mitotic index of human hepatoma cells decreased re markably, and the cellular growth inhibitory rate amounted to 52.4 %. The i ndices related with cell malignancy were alleviated significantly, such as the cell surface charge decreased significantly, the electrophoresis rate d ropped from 1.6 to 0.8 mum . s(-1). V-1 . cm(-1), the average value of alph a -fetoprotein (alpha -FP) content decreased from 314 to 95 mug/g (protein) , and gamma -glutamyl-transpeptidase (gamma -GT) activity from 0.9 to 0.14 U/g (protein). The cell differentiation index increased significantly, such as the average levels of tyrosine-alpha -ketoglutarate transaminase (TAT) activity increased from 11.6 to 36 mu mol/g ( protein), and the colonogenic potential decreased by 95.6%. The apoptotic bodies, detached cells, and ap optotic morphological features appeared, and the treated cells' DNA was fra gmented as observed by the comet assay. The flow cytometric results showed that a 42.9% fractional DNA content existed in the treated cells. CONCLUSIO N: DDC can inhibit human hepatoma cells proliferation, and can induce redif ferentiation as well as apoptosis. AM: To examine the effects of diethyldit hiocarbamate DDC on the proliferation, redifferentiation, and apoptosis in human hepatoma cells.