Statistical optimization of bioluminescence of Photobacterium phosphoreum KCTC2852

Citation
Bs. Lee et al., Statistical optimization of bioluminescence of Photobacterium phosphoreum KCTC2852, J BIOSCI BI, 92(1), 2001, pp. 72-76
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
92
Issue
1
Year of publication
2001
Pages
72 - 76
Database
ISI
SICI code
1389-1723(200107)92:1<72:SOOBOP>2.0.ZU;2-D
Abstract
In this study, media optimization by statistically designed experiments sti mulated an increase in the intensity and stability of bioluminescence of Ph otobacterium phosphoreum KCTC 2852 during continuous cultivation. The Plack ett-Burman design method selected two components (NaCl, yeast extract) amon g nine components of the seawater complete (SC) medium. The response-surfac e design method calculated the optimum concentration of NaCl (31.1 g/l) and yeast extract (5.1 g/l). In the optimized medium, bioluminescence per cell increased twofold. During continuous cultivation in a stirred tank reactor , bioluminescence decreased rapidly in the SC medium, whereas the optimized medium could maintain bioluminescence for more than 80 h, resulting in an increased bioluminescence. Upon contact with 0.1 ppm Hg2+, a sharp decrease in bioluminescence was observed, which was reproducible in the measuring c hamber. This shows the possibility that P. phosphoreum could be cultivated continuously with enhanced bioluminescence stability and that on-line water toxicity could be monitored continuously.