FISH examination of lymphocytes from Mayak workers for assessment of translocation induction rate under chronic radiation exposures

Citation
Le. Burak et al., FISH examination of lymphocytes from Mayak workers for assessment of translocation induction rate under chronic radiation exposures, INT J RAD B, 77(8), 2001, pp. 901-908
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
ISSN journal
0955-3002 → ACNP
Volume
77
Issue
8
Year of publication
2001
Pages
901 - 908
Database
ISI
SICI code
0955-3002(200108)77:8<901:FEOLFM>2.0.ZU;2-3
Abstract
Purpose: To estimate the translocation-induction rate under chronic exposur e conditions by measuring chromosome aberration frequencies in lymphocytes from Mayak nuclear workers using fluorescence in situ hybridization (FISH). Materials and methods: Lymphocytes were examined from 27 nuclear workers at the Mayak Production Association and two control individuals using FISH wi th probes for chromosomes 1, 2 and 4. Official doses derived from worker fi lm-badge records varied from 0 to 8.50 Gy. Results: The mean (+/- SD) genome-equivalent translocation frequency (F-G) was 2.30 (+/-0.75)% in the zero-dose group (n=7), and Poisson regression an alysis provided the best-fit equation of F-G(%)=2.96 (+/-0.39) +0.69 (+/-0. 14)D+0.12(+/-0.05)A, where D is the film-badge-derived dose (Gy), and A is age centred at 67 years. The induction rate would increase to nearly 1% Gy( -1) if the radiation dose to bone marrow, one of the major organs for lymph ocytes and where their precursor cells reside, is considered. Conclusion: The estimated induction rate in vivo appeared substantially sma ller than linear coefficients estimated from various in vitro studies.