Time-dose relationships in radiation-enhanced integration

Citation
Cw. Stevens et al., Time-dose relationships in radiation-enhanced integration, INT J RAD B, 77(8), 2001, pp. 841-846
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
ISSN journal
0955-3002 → ACNP
Volume
77
Issue
8
Year of publication
2001
Pages
841 - 846
Database
ISI
SICI code
0955-3002(200108)77:8<841:TRIRI>2.0.ZU;2-9
Abstract
Purpose: We have Shown that, ionizing radiation increases recombination, as manifested by increased Stable transduction of both plasmid and adenoviral vectors. This paper reports the duration of increased recombination after irradiation. Materials and methods: A549 or NIH/3T3 cells were transfected at various ti mes after irradiation. Cells were also irradiated with several fractionatio n schemes and then transfected. Results: Enhanced integration (El) is a very long-lived process, lasting at least 2-3 days after single radiation fractions. The duration of EI activa tion is radiation dose-dependent. The efficiency of EI is dependent on radi ation close and independent of fractionation, such that low close-rate, fra ctionated and single radiation doses result in similar levels of EI when co rrected for differences in cytotoxicity. Conclusions: Radiation, given with fraction sizes and dose-rates used in cl inical radiation therapy, induces a Ion-lived hyper-recombination state, Si nce radiotherapy is already a component of treatment for many malignancies and is integrated into radiation-gene therapy trials, an understanding of r ecombination events that. improve gene delivery is important and timely.