Monitoring of methyl jasmonate-responsive genes in Arabidopsis by cDNA macroarray: Self-activation of jasmonic acid biosynthesis and crosstalk with other phytohormone signaling pathways

Citation
Y. Sasaki et al., Monitoring of methyl jasmonate-responsive genes in Arabidopsis by cDNA macroarray: Self-activation of jasmonic acid biosynthesis and crosstalk with other phytohormone signaling pathways, DNA RES, 8(4), 2001, pp. 153-161
Citations number
47
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
DNA RESEARCH
ISSN journal
1340-2838 → ACNP
Volume
8
Issue
4
Year of publication
2001
Pages
153 - 161
Database
ISI
SICI code
1340-2838(20010831)8:4<153:MOMJGI>2.0.ZU;2-U
Abstract
Jasmonates mediate various physiological events in plant cells such as defe nse responses, flowering, and senescence through intracellular and intercel lular signaling pathways, and the expression of a large number of genes app ears to be regulated by jasmonates. In order to obtain information on the r egulatory network of jasmonate-responsive genes (JRGs) in Arabidopsis thali ana (Arabidopsis), we screened 2880 cDNA clones for jasmonate responsivenes s by a cDNA macroarray procedure. Since many of the JRGs reported so far ha ve been identified in leaf tissues, the cDNA clones used were chosen from a non-redundant EST library that was prepared from above-ground organs. Hybr idization to the filters was achieved using alpha-P-33-labeled single-stran d DNAs synthesized from mRNAs obtained from methyl jasmonate (MeJA)-treated and untreated Arabidopsis seedlings. Data analysis identified 41 JRGs whos e mRNA levels were changed by more than three fold in response to MeJA. Thi s was confirmed by Northern blot analysis by using eight representatives. A mong the 41 JRGs identified, 5 genes were JA biosynthesis genes and 3 genes were involved in other signaling pathways (ethylene, auxin, and salicylic acid). These results suggest the existence of a positive feedback regulator y system for JA biosynthesis and the possibility of crosstalk between JA si gnaling and other signaling pathways.