Differentiation of mouse hepatitis viruses in animal facilities in Japan by use of nucleotide analysis of the nucleocapsid gene

Citation
Yk. Yamada et al., Differentiation of mouse hepatitis viruses in animal facilities in Japan by use of nucleotide analysis of the nucleocapsid gene, COMPAR MED, 51(4), 2001, pp. 319-325
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Animal Sciences","Animal & Plant Sciences
Journal title
COMPARATIVE MEDICINE
ISSN journal
1532-0820 → ACNP
Volume
51
Issue
4
Year of publication
2001
Pages
319 - 325
Database
ISI
SICI code
1532-0820(200108)51:4<319:DOMHVI>2.0.ZU;2-X
Abstract
The nucleotide sequences of the coding region of the nucleocapsid (N) gene of 12 mouse hepatitis virus (MHV) strains recently found in animal faciliti es in Japan were analyzed. Nucleotide sequencing was performed directly on polymerase chain reaction (PCR) products amplified by reverse transcription (RT) and polymerase chain reaction (RT-PCR) analysis from fecal samples or isolated viruses. Phylogenetic analysis of these MHV strains along with th ose reported previously indicated that sequence analysis of the N gene was a useful tool for differentiation of MHV strains, although most MHV strains in Japanese facilities were phylogenetically close. Results suggested that interchange of mice infected with MHV among facilities provided opportunit ies of introduction of MHV into otherwise MHV-free facilities and that the source of MHV infection could be traced by use of nucleotide analysis of th e N gene.