Cadmium-induced abnormality in strains of Euglena gracilis: morphological alteration and its prevention by zinc and cyanocobalamin

Citation
M. Watanabe et T. Suzuki, Cadmium-induced abnormality in strains of Euglena gracilis: morphological alteration and its prevention by zinc and cyanocobalamin, COMP BIOC C, 130(1), 2001, pp. 29-39
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN journal
1532-0456 → ACNP
Volume
130
Issue
1
Year of publication
2001
Pages
29 - 39
Database
ISI
SICI code
1532-0456(200109)130:1<29:CAISOE>2.0.ZU;2-4
Abstract
Euglena gracilis is susceptible to cadmium (Cd) at high concentrations. The re are no comparative data on cytotoxicity or abnormality of CdCl2 to E. gr acilis Z and its achlorophyllous mutant SMZ. The present study examined the cytotoxicity of CdCl2 under continual exposure at levels ranging from sub- ppm to ppm, and assessed the effects of zinc (Zn) or cyanocobalamin (VB,,) supplementation on the suppression of Cd-induced abnormal cell proliferatio n and hypertrophy. With Zn levels restricted to I ppm [as Zn++], cell growt h of both E. gracilis strains was reduced in proportion to Cd concentration . More abnormal cells (hypertrophied, V-shape and starfish-shape) were obse rved in both strains at sub-ppm levels of Cd. ZnSO4 supplementation from 2 to 63 ppm significantly suppressed the incidence of Cd-induced abnormality. However, a significant increase in abnormal cells was observed following Z n supplementation at levels of 125 and 250 ppm, which produced remarkable d ifferences in cell morphology. The incidence of abnormal cells varied with supplemented VB12 levels ranging from 4 to 250 ppb in both E. gracilis stra ins. (C) 2001 Elsevier Science Inc. All rights reserved.