Comparative evaluation of laser-based microarray scanners

Ramdas, L Wang, J Hu, L Cogdell, D Taylor, E Zhang, W

L. Ramdas et al., Comparative evaluation of laser-based microarray scanners, BIOTECHNIQU, 31(3), 2001, pp. 546

7

INGLESE

Article

Biochemistry & Biophysics

BIOTECHNIQUES

0736-6205 → ACNP

31

3

2001

ISI

0736-6205(200109)31:3<546:CEOLMS>2.0.ZU;2-G

Laboratories use different laser-based scanners to scan microarray images. To assess whether results from different scanners are comparable, and thus whether data from different laboratories can be compared, we scanned the sa me microarray slide with three, commercial scanners that use different imag ing techniques. After the acquisition of the microarray images produced by the three scanners, the images were quantified using a single imaging softw are package and protocol The results were compared, and we found that the d ata obtained from the three scanners were comparable and that the variation s. caused by the use of different instruments were negligible, in spite of the fact that the scanners were based on different optical imaging techniqu es.