Comparative evaluation of laser-based microarray scanners

Citation
L. Ramdas et al., Comparative evaluation of laser-based microarray scanners, BIOTECHNIQU, 31(3), 2001, pp. 546
Citations number
7
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOTECHNIQUES
ISSN journal
0736-6205 → ACNP
Volume
31
Issue
3
Year of publication
2001
Database
ISI
SICI code
0736-6205(200109)31:3<546:CEOLMS>2.0.ZU;2-G
Abstract
Laboratories use different laser-based scanners to scan microarray images. To assess whether results from different scanners are comparable, and thus whether data from different laboratories can be compared, we scanned the sa me microarray slide with three, commercial scanners that use different imag ing techniques. After the acquisition of the microarray images produced by the three scanners, the images were quantified using a single imaging softw are package and protocol The results were compared, and we found that the d ata obtained from the three scanners were comparable and that the variation s. caused by the use of different instruments were negligible, in spite of the fact that the scanners were based on different optical imaging techniqu es.