Reactivity of autoantibodies of autoimmune hemolytic anemia with recombinant rhesus blood group antigens or anion transporter band3

Citation
S. Iwamoto et al., Reactivity of autoantibodies of autoimmune hemolytic anemia with recombinant rhesus blood group antigens or anion transporter band3, AM J HEMAT, 68(2), 2001, pp. 106-114
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
AMERICAN JOURNAL OF HEMATOLOGY
ISSN journal
0361-8609 → ACNP
Volume
68
Issue
2
Year of publication
2001
Pages
106 - 114
Database
ISI
SICI code
0361-8609(200110)68:2<106:ROAOAH>2.0.ZU;2-L
Abstract
The specificity of autoantibodies in autoimmune hemolytic anemia (AlHA) has been studied using the serological procedure and immunoprecipitation techn ique with rare phenotype red cells. We attempted to analyze specificity usi ng recombinant rhesus (Rh) blood group and band3 antigens expressed on eryt hroleukemic cell lines, KU812E. The autoantibody eluates were isolated by t he acid elution procedure from the red cells of 20 ANA patients. The recomb inant Rh antigens, RhD, cE, ce, CE, and chimera antigens CE-D and D-CE, wer e obtained by retroviral cDNA transduction into KU812E cells, and the cell line expressing the antigens was cloned. Band3 cDNA was also obtained and i ntroduced into KU812E and cloned KU812 expressing RhcE. The reactivities of ANA eluates with recombinant Rh and band3 antigens were studied by flow cy tometry. Fifteen eluates reacted with at least one of the RhcE, ce, or CE a ntigens, and four eluates reacted with RhD. Seven eluates with strong Rh sp ecificity were studied further using chimera antigen. Five eluates showed r educed or lost reactivity, although two eluates reacted identically with th e chimera antigens as wild type. These results indicated that conformationa l epitopes constituted by RhD or CE specific exofacial peptide loops are im portant for autoantibodies in most cases. Seven eluates reacted with band3, five exclusively, The coexpression study of RhcE and band3 did not enhance the expression of either antigen nor the reactivity with patient eluates, indicating that association of Rh and band3 was not involved in the appeara nce of autoantigen. Am. J. Hematol. 68:106-114, 2001. (C) 2001 Wiley-Liss, Inc.